Composite

Part:BBa_K3766109:Design

Designed by: Kamyar Keshavarz-Farajkhah   Group: iGEM21_Evry_Paris-Saclay   (2021-09-25)


pTet evoCDA1-BE4max T7RNAP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 706
    Illegal PstI site found at 1389
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1389
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 706
    Illegal PstI site found at 1389
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 706
    Illegal PstI site found at 1389
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

to do


Source

DNA synthesis, PCR and assembly

References